GDF15 Protein

Catalog No. ABIN6699908

Supplier Product No.: 009-001-u95-0005

Product Details

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Supplier Product No.: 009-001-u95-0005

Supplier: Rockland

Purpose: Human Growth and Differentiation Factor-15 Recombinant Protein

Purification: Growth and Differentiation Factor-15 purity was determined to be greater than 95% as determined by analysis by HpLC, UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Purity: 95,00%

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the inhibition of DU-145 cells and is typically 1-2 μg/mL.

Application Details

Application Notes:

Other: User Optimized

Application_Note: Growth and Differentiation Factor-15 Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Growth and Differentiation Factor-15 in immunological assays.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 50μL

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Alternative Name: GDF15 (GDF15 Products)

Background:

Synonyms: MIC-1, Placental TGFβ, Prostate differentiation factor

Background: Growth and Differentiation Factor 15 (GDF-15) is a TGFβ family member, made by the placenta and heart tissues, that has a role in regulating inflammatory and apoptotic pathways. GDF-15 has become an immerging marker of early heart disease and has the potential as being used as a molecule for screening patients for early heart failure. Recombinant human GDF-15 is a non-glycosylated, disulfide linked homodimer, containing two identical 113 amino acid chains, with a total molecular weight of 24.5 kDa.

UniProt: Q99988

Pathways: SARS-CoV-2 Protein Interactome