ADIPOQ Protein

Catalog No. ABIN6699890

Supplier Product No.: 009-001-u90-0005

Product Details

Target: ADIPOQ (Adiponectin (ADIPOQ))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Supplier Product No.: 009-001-u90-0005

Supplier: Rockland

Purpose: Human globular ACRP-30 Recombinant Protein

Purification: globular AC-30 purity was determined to be greater than 98% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Purity: 98,00%

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the ability to inhibit the proliferation of mouse M1 cells and is typically 1.0 - 2.5 μg/mL.

Application Details

Application Notes:

Other: User Optimized

Application_Note: Human globular ACRP-30 Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Human globular ACRP-30 in immunological assays.

Comment:

Suggested_Applications: Cellular Assay

Other_Performance_Data:

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 5 μL (5-50 μL)

Buffer: Lyophilized in 10 mM sodium phosphate, 0.75 mM DTT, pH 8.0.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: ADIPOQ (Adiponectin (ADIPOQ))

Alternative Name: ADIPOQ (ADIPOQ Products)

Background:

Synonyms: Adiponectin, Adipose most abundant gene transcript 1 protein (apm-1), Adipocyte complement-related 30 kDa protein (ACRP30), adipolean, Gelatin-binding protein

Background: Globular ACRP-30, gACRP-30, is a naturally occurring cleavage product of adiponectin, a molecule made exclusively by adipocytes. gACRP-30 is detected a relatively high concentrations in the serum and is thought to play an important role in hyperglycemia and insulin resistance. gACRP-30 signals through receptors, AdipoR1 and AdipoR2. Recombinant human gACRP-30 is a non-glycosylated protein, containing 145 amino acids, with a molecular weight of 16.7 kDa.

UniProt: Q15848