FLT3LG Protein

Catalog No. ABIN6699875

Supplier Product No.: 010-001-u86-0010

Product Details

Target: FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Supplier Product No.: 010-001-u86-0010

Supplier: Rockland

Purpose: Mouse Flt-3 Ligand Recombinant Protein

Purification: Flt-3 Ligand purity was determined to be greater than 98% as determined by analysis by reducing and non-reducing SDS-pAGE.

Purity: 98,00%

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent proliferation of mouse AML5 cells and is typically 5-8 ng/mL.

Application Details

Application Notes:

Other: User Optimized

Application_Note: Flt-3 Ligand Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti- Flt-3 Ligand in immunological assays.

Comment:

Suggested_Applications: Cellular Assay, IHC, WB

Other_Performance_Data:

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, pH 7.5

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

References

Turnis, Kaminska, Smith, Kartchner, Vogel, Laxton, Ashmun, Ney, Opferman: "Requirement for antiapoptotic MCL-1 during early erythropoiesis." in: Blood, Vol. 137, Issue 14, pp. 1945-1958, (2021) (PubMed).

Target Details

Target: FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

Alternative Name: Flt3lg (FLT3LG Products)

Background:

Synonyms: FLt3 L, Fms-related tyrosine kinase 3 ligand

Background: FMS-related Tyrosine Kinase 3 Ligand (FLT-3 Ligand) is a growth factor important for the proliferation of hematopoietic cells. FLT-3 Ligand binds to, and transmits signals through, the receptor tyrosine kinase known as FMS-like Tyrosine Kinase-3 (FLT-3). FLT-3 Ligand promotes long-term expansion and differentiation of human pro-B cells in the presence of IL-7 or in combination of IL-7 and IL-3. Human FLT-3 Ligand can stimulate the proliferation of cells expressing murine FLT-3 receptors. Recombinant mouse FLT-3 Ligand is a non-glycosylated protein, containing 163 amino acids, with a molecular weight of 18.6 kDa.

UniProt: P49772

Pathways: RTK Signaling