FGF22 Protein

Catalog No. ABIN6699854

Supplier Product No.: 009-001-u78-0005

Product Details

Target: FGF22 (Fibroblast Growth Factor 22 (FGF22))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Supplier Product No.: 009-001-u78-0005

Supplier: Rockland

Purpose: Human Fibroblast Growth Factor-22 Recombinant Protein

Purification: Fibroblast Growth Factor-22 purity was determined to be greater than 97% as determined by analysis by HpLC, UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Purity: 97,00%

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity, as determined by the dose-dependent proliferation of 4MBr-5 cells, is typically 50-300 ng/mL.

Application Details

Application Notes:

Other: User Optimized

Application_Note: Fibroblast Growth Factor-22 Recombinant Protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-Fibroblast Growth Factor-22 in immunological assays.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 5 μL (5-50 μL)

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: FGF22 (Fibroblast Growth Factor 22 (FGF22))

Alternative Name: FGF22 (FGF22 Products)

Background:

Synonyms: FGFM

Background: Fibroblast Growth Factors (FGFs) are a 22 member family of proteins known to be involved in angiogenesis, wound healing and embryonic development. As a family, they bind to heparin and signal through four receptor tyrosine kinases called, FGFR1, 2, 3 and 4. Human FGF-22 is a member of the FGF-7 subfamily and is synthesized by multiple cell lines including neurons, keratinocytes and skeletal muscle myotubes. Human FGF-22 shares 86 % homology with mouse FGF-22. Recombinant human FGF-22 is a non-glycosylated protein, containing149 amino acids, with a molecular weight of 17.3 kDa.

UniProt: Q9HCT0

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway