FGF1 Protein
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- Target See all FGF1 Proteins
- FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Supplier Product No.
- 009-001-u79-0010
- Supplier
- Rockland
- Purpose
- Human Fibroblast Growth Factor acidic Recombinant Protein
- Purification
- Fibroblast Growth Factor acidic purity was determined to be greater than 97% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
- Purity
- 97,00%
- Endotoxin Level
- Measured by LAL is typically ≤ 1 EU/μg protein.
- Biological Activity Comment
- The activity is determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells and is typically 2.0 ng/mL.
- Top Product
- Discover our top product FGF1 Protein
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- Application Notes
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Other: User Optimized
Application_Note: Fibroblast Growth Factor acidic Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Fibroblast Growth Factor acidic in immunological assays.
- Comment
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Suggested_Applications: Cellular Assay
Other_Performance_Data:
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 10 μL (10-100 μL)
- Buffer
- Buffer formation: 10 mM sodium phosphate, 150 mM sodium sulfate, pH 7.5.
- Preservative
- Without preservative
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- Expiry Date
- 6 months
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- Target
- FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))
- Alternative Name
- FGF1 (FGF1 Products)
- Background
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Synonyms: Heparin-binding growth factor 1 (HBGF-1), Beta-endothelial growth factor, ECGF-beta, acidic fibroblast growth factor (aFGF)
Background: Fibroblast Growth Factors (FGFs) are a 22 member family of proteins known to be involved in angiogenesis, wound healing and embryonic development. As a family, they bind to heparin and signal through four receptor tyrosine kinases called, FGFR1, 2, 3 and 4. FGF-acidic, or FGF-1, is a particularly potent inducer of DNA synthesis and has chemotactic activities. Recombinant human FGF acidic is a non-glycosylated protein, containing 141 amino acids, with a molecular weight of 16 kDa.
- UniProt
- P05230
- Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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