EBI3 Protein

Catalog No. ABIN6699803

Supplier Product No.: 009-001-b66-0005

Product Details

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Supplier Product No.: 009-001-b66-0005

Supplier: Rockland

Purpose: Human Epstein-Barr Virus Induced Gene 3 Recombinant Protein

Purification: Epstein-Barr Virus Induced Gene 3 purity was determined to be greater than 90% as determined by reducing and non-reducing SDS-pAGE.

Purity: 90,00%

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: Human EBI3 has no known independent biological function, but is assayed by qualitative binding to an anti-EBI-3 antibody.

Application Details

Application Notes:

Other: User Optimized

Application_Note: Epstein-Barr Virus Induced Gene 3 Recombinant Protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-Epstein-Barr Virus Induced Gene 3 in immunological assays.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 5 μL (5-50 μL)

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: 0.5 % Mannitol

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Alternative Name: EBI3 (IL-27b Products)

Background:

Synonyms: IL-35/EBI3, IL-27/EBI3

Background: Epstein-Barr Virus Induced Gene-3 (EBI3), is a secreted glycoprotein belonging to the hematopoietin receptor family and is related to the p40 subunit of IL-12. EBI3 was identified by its induced expression in B-lymphocytes in response to Epstein-Barr virus infection. EBI3 forms heterodimers with p28 to form IL-27 and with p35 to form IL-35. Both IL-27 and IL-35 have anti-inflammatory and regulatory activity. Recombinant human EBI3 is a non-glycosylated protein, containing 210 amino acids, with a molecular weight of 23.3 kDa.

UniProt: Q14213