Cyclin O Protein (CCNO) (GST tag)
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- Target See all Cyclin O (CCNO) Proteins
- Cyclin O (CCNO)
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Insect cells (Sf9)
- Purification tag / Conjugate
- This Cyclin O protein is labelled with GST tag.
- Application
- Western Blotting (WB)
- Supplier Product No.
- 009-001-r82s
- Supplier
- Rockland
- Purpose
- CyclinO recombinant protein-GST fusion protein
- Purification
- Recombinant full-length human CyclinO was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.
- Purity
- >95%
- Top Product
- Discover our top product CCNO Protein
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- Application Notes
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Western_Blot_Dilution: User Optimized
Application_Note: CyclinO Protein is suitable for use in Western Blot. Expect a band approximately ~ 69 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.1 μg/μL
- Buffer
- CyclinO Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- Expiry Date
- 12 months
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- Target
- Cyclin O (CCNO)
- Alternative Name
- CCNO (CCNO Products)
- Background
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Synonyms: CCNO, CCNU, UDG2, Cyclin-O
Background: Cyclin O belongs to the highly conserved cyclin family. These family members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclin O plays an important role in the processes of transcription. Cyclin O also plays an important role in DNA repair and cell cycle progression. Cyclin O localizes exclusively in the nucleus (1). Cyclin O shows significant uracil-DNA glycosylase activity in which the activity of in vitro translated UDG2 was not as robust as that of the mitochondrial isoform of UNG (2). Cyclin O Protein is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Cancer, and Cell Cycle research.
- NCBI Accession
- NM_021147
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