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Cyclin H Protein (CCNH) (GST tag)

Rockland 009-001-r80s CCNH Origin: Human Host: Insect cells (Sf9) Recombinant >90% WB
Rockland
Catalog No. ABIN6699745
Supplier Product No.: 009-001-r80s
  • Target See all Cyclin H (CCNH) Proteins
    Cyclin H (CCNH)
    Protein Type
    Recombinant
    Origin
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Source
    • 4
    • 3
    • 2
    • 2
    • 1
    Insect cells (Sf9)
    Purification tag / Conjugate
    This Cyclin H protein is labelled with GST tag.
    Application
    Western Blotting (WB)
    Supplier Product No.
    009-001-r80s
    Supplier
    Rockland
    Purpose
    CyclinH recombinant protein-GST fusion protein
    Purification
    Recombinant full length human CyclinH was expressed in insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.
    Purity
    >90%
    Top Product
    Discover our top product CCNH Protein
  • Application Notes

    Western_Blot_Dilution: User Optimized

    Application_Note: CyclinH Protein is suitable for use in Western Blot. Expect a band approximately ~ 39 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.1 μg/μL
    Buffer
    CyclinH Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
    Storage
    -80 °C
    Storage Comment
    Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
    Expiry Date
    12 months
  • Target
    Cyclin H (CCNH)
    Alternative Name
    CCNH (CCNH Products)
    Background

    Synonyms: CCNH, CAK, p34, p37, Cyclin-H, MO15-associated protein

    Background: Cyclin H is related to the highly conserved cyclin family. Cyclin family members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Cyclin H forms a complex with CDK7 kinase and the ring finger protein MAT1 which is able to phosphorylate CDK2 and CDC2 kinases and thus act as a CDK-activating kinase (CAK) (1). Cdk-activating kinase complex is a component of human transcription factor TFIIH and plays an important role in the processes of transcription, DNA repair, and cell cycle progression (2). Cyclin H Protein is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Cancer, and Cell Cycle research.

    NCBI Accession
    NM_001239
    Pathways
    Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase
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