Cyclin C Protein (CCNC) (GST tag)

Catalog No. ABIN6699737

Supplier Product No.: 009-001-r77s

Product Details

Target: Cyclin C (CCNC)

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Purification tag / Conjugate: This Cyclin C protein is labelled with GST tag.

Application: Western Blotting (WB)

Supplier Product No.: 009-001-r77s

Supplier: Rockland

Purpose: CyclinC recombinant protein-GST fusion protein

Purification: Recombinant full-length human CyclinC was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >70% by densitometry.

Purity: >70%

Application Details

Application Notes:

Western_Blot_Dilution: User Optimized

Application_Note: CyclinC Protein is suitable for use in Western Blot. Expect a band approximately ~ 55 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.05 μg/μL

Buffer: CyclinC Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: Cyclin C (CCNC)

Alternative Name: CCNC (CCNC Products)

Background:

Synonyms: CCNC, CycC, Cyclin-C, SRB11 homolog, hSRB11

Background: Cyclin C is a member of the cyclin family of proteins and interacts with the cyclin-dependent kinase 8 (CDK8) and induces the phosphorylation of the carboxy-terminal domain of the large subunit of RNA polymerase II. The mRNA levels of Cyclin C fluctuate during the cell cycle and peak at the G1 phase. Cyclin C acts as a critical regulator of the G0/G1 transition of human Hematopoietic stem cells (1). Cyclin C can also combine with CDK3 to stimulate Rb phosphorylation at S807/811 during the G0/G1 transition, and this phosphorylation is required for cells to exit G0 efficiently (2). Cyclin C Protein is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Cancer, and Cell Cycle research.

NCBI Accession: NM_005190

Pathways: Cell Division Cycle, Regulation of Lipid Metabolism by PPARalpha