CTGF Protein

Catalog No. ABIN6699724

Supplier Product No.: 009-001-u72-0005

Product Details

Target: CTGF (Connective Tissue Growth Factor (CTGF))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Supplier Product No.: 009-001-u72-0005

Supplier: Rockland

Purpose: Human Connective Tissue Growth Factor Recombinant Protein

Purification: Connective Tissue Growth Factor purity was determined to be greater than 98% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Purity: 98,00%

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent stimulation of HUVEC proliferation and is typically 1-2 μg/mL.

Application Details

Application Notes:

Other: User Optimized

Application_Note: Connective Tissue Growth Factor Recombinant Protein has been tested by SDS-PAGE and biological assay and is suitable as a control for polyclonal or monoclonal anti-Connective Tissue Growth Factor in immunological assays.

Comment:

Suggested_Applications: Cellular Assay

Other_Performance_Data:

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 5 μL (5-50 μL)

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: CTGF (Connective Tissue Growth Factor (CTGF))

Alternative Name: CTGF (CTGF Products)

Background:

Synonyms: CCN2, HCS24, IGFBP8

Background: Connective Tissue Growth Factor (CTGF) is a member of cysteine rich regulatory proteins that are both mitogenic and chemotactic. Each protein has an Insulin-like Growth Factor (IGF)-binding domain, a thrombospondin type 1 domain and cysteine knot region. CTGF has multiple effects on development and differentiation. Recombinant human CTGF is a non-glycosylated protein, containing 98 amino acids, with a molecular weight of 11.2 kDa.

UniProt: P29279

Pathways: Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Growth Factor Binding