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G-CSF Protein

Rockland 010-001-u93-0010 CSF3 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant 98,00% SDS
Rockland
Catalog No. ABIN6699721
Supplier Product No.: 010-001-u93-0010
  • Target See all G-CSF (CSF3) Proteins
    G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
    Protein Type
    Recombinant
    Origin
    • 21
    • 7
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Mouse
    Source
    • 18
    • 10
    • 5
    • 3
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Application
    SDS-PAGE (SDS)
    Supplier Product No.
    010-001-u93-0010
    Supplier
    Rockland
    Purpose
    Mouse Granulocyte Colony Stimulating Factor Recombinant Protein
    Purification
    Granulocyte Colony Stimulating Factor purity was determined to be greater than 98% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
    Purity
    98,00%
    Endotoxin Level
    Measured by LAL is typically ≤ 1 EU/μg protein.
    Biological Activity Comment
    The activity is determined by the dose-dependent proliferation of mouse M-NFS-60 cells and is typically less than 10-60 ng/mL.
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  • Application Notes

    Other: User Optimized

    Application_Note: Granulocyte Colony Stimulating Factor Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Granulocyte Colony Stimulating Factor in immunological assays.

    Comment

    Suggested_Applications: Cellular Assay

    Other_Performance_Data:

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution_Buffer: Restore with deionized water (or equivalent)

    Reconstitution_Volume: 10 μL (10-100 μL)

    Buffer

    Buffer: 0.01 M Sodium Citrate, pH 3.0

    Stabilizer: None

    Preservative
    Without preservative
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
    Expiry Date
    6 months
  • Target
    G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
    Alternative Name
    Csf3 (CSF3 Products)
    Background

    Synonyms: CSF-3, MGI-1G, GM-CSFβ, pluripoietin

    Background: Granulocyte Colony-Stimulating Factor, or G-CSF, is a growth factor that is considered the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, IL-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF, while in epithelial, endothelial, and fibroblastic cells secretion of G-CSF is induced by IL-17. Human and mouse G-CSF are cross-reactive. Recombinant mouse G-CSF is a non-glycosylated protein, containing 179 amino acids, with a molecular weight of 19 kDa.

    UniProt
    P09920
    Pathways
    Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
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