Cofilin Protein (His tag)
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- Target See all Cofilin (CFL1) Proteins
- Cofilin (CFL1) (Cofilin 1 (CFL1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Cofilin protein is labelled with His tag.
- Application
- Western Blotting (WB)
- Supplier Product No.
- 009-001-r72s
- Supplier
- Rockland
- Purpose
- Cofilin 1 recombinant protein-HIS Epitope
- Purification
- Recombinant full-length human Cofilin 1 was expressed in E. coli cells using an N-Terminal his epitope. The purity was determined to be >95% by densitometry.
- Purity
- >95%
- Top Product
- Discover our top product CFL1 Protein
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Cofilin 1 (CFL1) (AA 2-166) protein (His tag)
CFL1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Cofilin 1 (CFL1) (AA 1-166) protein (GST tag)CFL1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Cofilin 1 (CFL1) (AA 1-166) protein (His tag)CFL1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
Cofilin 1 (CFL1) protein (Myc-DYKDDDDK Tag)CFL1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Western_Blot_Dilution: User Optimized
Application_Note: Cofilin 1 Protein is suitable for use in Western Blot. Expect a band approximately ~ 23 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 μg/μL
- Buffer
- Cofilin 1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- Expiry Date
- 12 months
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- Target
- Cofilin (CFL1) (Cofilin 1 (CFL1))
- Alternative Name
- CFL1 (CFL1 Products)
- Background
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Synonyms: CFL1, CFL, Cofilin-1, 18 kDa phosphoprotein, p18, Cofilin, non-muscle isoform
Background: Cofilin 1 (non-muscle), also known as CFL1, is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH -dependent manner (1). It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus (2). CFL1 processed proteins are most probably produced by HIV-1 protease digestion. Cofilin 1 Protein is ideal for investigators involved in Signaling Proteins, Microtubule/Actin Associated Proteins, Cancer, Cardiovascular Disease, Invasion/Metastasis, and Neurobiology research.
- NCBI Accession
- NM_005507
- Pathways
- Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events
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