Q53X90 Protein
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- Target
- Q53X90
- Protein Type
- Recombinant
- Origin
- Human
- Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Supplier Product No.
- 009-001-u66-0005
- Supplier
- Rockland
- Purpose
- Human Beta-cell Attracting Chemokine 1 (CXCL13) Recombinant Protein
- Purification
- Beta-cell Attracting Chemokine 1 (CXCL13) purity was determined to be greater than 97% as determined by analysis by HpLC, UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.
- Purity
- 97,00%
- Endotoxin Level
- Measured by LAL is typically ≤ 1 EU/μg protein.
- Biological Activity Comment
- The activity is determined by its ability to chemoattract BaF3 cells transfected with CXCR5 and is typically 5-20 ng/mL.
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- Application Notes
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Other: User Optimized
Application_Note: Beta-cell Attracting Chemokine 1 Recombinant Protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-Beta-cell Attracting Chemokine 1 in immunological assays.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
-
Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 5 μL (5-50 μL)
- Buffer
-
Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
- Preservative
- Without preservative
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- Expiry Date
- 6 months
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- Target
- Q53X90
- Background
-
Synonyms: CXCL13, BLC, BLR1 Ligand
Background: B cell Attracting Chemokine-1 (BCA-1), also known as CXCL13, is expressed at high levels in lymphoid tissues such as the spleen, lymph nodes and Peyer's patches. It has been shown to activate signaling through the receptor BLR1 (Burkitt's lymphoma receptor 1) to chemoattract B cells. Recombinant human BCA-1 is a non-glycosylated protein, containing 87 amino acids, with a molecular weight of 10.3 kDa.
- UniProt
- Q53X90
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