CXCL10 Protein

Catalog No. ABIN6699670

Supplier Product No.: 009-001-v69-0005

Product Details

Target: CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Supplier Product No.: 009-001-v69-0005

Supplier: Rockland

Purpose: Human IP-10 (CXCL10) Recombinant Protein

Purification: Ip-10 (CXCL10) purity was determined to be greater than 97% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Purity: 97,00%

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by its ability to chemoattract human T lymphocytes at 5 - 40 ng/mL.

Application Details

Application Notes:

Other: User Optimized

Application_Note: IP-10 Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-IP-10 in immunological assays.

Comment:

Suggested_Applications: Cellular Assay

Other_Performance_Data:

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 5 μL (5-50 μL)

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

Alternative Name: CXCL10 (CXCL10 Products)

Background:

Synonyms: 10 kDa interferon gamma-induced protein (IP-10), crg-2, small-inducible cytokine B10

Background: The chemokine IP-10 (or CXCL10) is a chemokine made by monocytes, endothelial cells and fibroblasts in response to treatment with IFNγ. IP-10 functions as a chemoattractant to cells (activated T cells and NK cells) expressing the G protein-coupled receptor, CXCR3. IP-10 plays an important role in Th1 type inflammatory diseases and autoimmune diseases such as, Hashimoto's thyroiditis, Graves' disease and Type 1 diabetes mellitus. Recombinant human IP-10 is a non-glycosylated protein, containing 77 amino acids, with a molecular weight of 8.5 kDa.

UniProt: P02778