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CD40 Ligand Protein (CD40LG)

Rockland 009-001-u68-0010 CD40LG Origin: Human Host: Escherichia coli (E. coli) Recombinant 98,00% SDS
Rockland
Catalog No. ABIN6699578
Supplier Product No.: 009-001-u68-0010
  • Target See all CD40 Ligand (CD40LG) Proteins
    CD40 Ligand (CD40LG)
    Protein Type
    Recombinant
    Origin
    • 42
    • 17
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Source
    • 35
    • 19
    • 10
    • 5
    • 5
    • 2
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Application
    SDS-PAGE (SDS)
    Supplier Product No.
    009-001-u68-0010
    Supplier
    Rockland
    Purpose
    Human CD40 Ligand Recombinant Protein
    Purification
    CD40 Ligand purity was determined to be greater than 98% as determined by analysis by HpLC, UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.
    Purity
    98,00%
    Endotoxin Level
    Measured by LAL is typically ≤ 1 EU/μg protein.
    Biological Activity Comment
    The activity is tested by HEK-blue ™ CD40L Activity. Acceptable Level <50ng/mL, >2.0x10^4 units/mg. Additionally testing by ELISA: IL-8 production by human PBMCs.
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    Discover our top product CD40LG Protein
  • Application Notes

    Other: User Optimized

    Application_Note: CD40 Ligand Recombinant Protein has been tested by SDS-PAGE and biological assay and is suitable as a control for polyclonal or monoclonal anti-CD40 Ligand in immunological assays.

    Comment

    Suggested_Applications: Cellular Assay

    Other_Performance_Data:

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution_Buffer: Restore with deionized water (or equivalent)

    Reconstitution_Volume: 10 μL (10-100 μL)

    Buffer

    Buffer: 0.01 M Sodium Phosphate, pH 7.5

    Stabilizer: None

    Preservative
    Without preservative
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
    Expiry Date
    6 months
  • Target
    CD40 Ligand (CD40LG)
    Alternative Name
    CD40LG (CD40LG Products)
    Background

    Synonyms: TNFSF5, TRAP, CD154, gp39, T-BAM

    Background: CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-α, and IL-2. Recombinant human CD40 Ligand is a non-glycosylated protein, containing 149 amino acids, with a molecular weight of 16.3 kDa.

    UniProt
    P29965
    Pathways
    NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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