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c-FOS Protein (GST tag)

Rockland 009-001-r95s c-Fos Origin: Human Host: Insect cells (Sf9) Recombinant >90% WB
Rockland
Catalog No. ABIN6699563
Supplier Product No.: 009-001-r95s
  • Target See all c-FOS (c-Fos) Proteins
    c-FOS (c-Fos) (c-Fos)
    Protein Type
    Recombinant
    Origin
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Source
    • 9
    • 2
    • 2
    • 2
    • 1
    • 1
    Insect cells (Sf9)
    Purification tag / Conjugate
    This c-FOS protein is labelled with GST tag.
    Application
    Western Blotting (WB)
    Supplier Product No.
    009-001-r95s
    Supplier
    Rockland
    Purpose
    FOS recombinant protein-GST fusion protein
    Purification
    Recombinant full-length human FOS was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.
    Purity
    >90%
    Top Product
    Discover our top product c-Fos Protein
  • Application Notes

    Western_Blot_Dilution: User Optimized

    Other: Kinase Assay-User Optimized

    Application_Note: FOS Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~78 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.2 μg/μL
    Buffer
    FOS Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
    Storage
    -80 °C
    Storage Comment
    Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
    Expiry Date
    12 months
  • Target
    c-FOS (c-Fos) (c-Fos)
    Alternative Name
    FOS (c-Fos Products)
    Background

    Synonyms: AP-1, c-FOS, Proto-oncogene c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7

    Background: FOS is a member of the FOS gene family that consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. FOS gene encodes a leucine zipper protein that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation (1). In some cases, expression of the FOS gene has also been associated with apoptotic cell death. FOS overexpression leads to decreased phosphorylation and dimerization of STAT1, which in turn downregulates p21 gene expression (2). This regulatory pathway may enhance the proliferation of lymphocytes in rheumatoid arthritis patients. FOS Protein is ideal for investigators involved in Signaling Proteins, Transcription Proteins, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Invasion/Metastasis, and Neurobiology research.

    NCBI Accession
    NM_005252
    Pathways
    S100 Proteins
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