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BDNF Protein

Rockland 009-001-c27 BDNF Origin: Human Host: Escherichia coli (E. coli) Recombinant 97,00% SDS
Rockland
Catalog No. ABIN6699554
Supplier Product No.: 009-001-c27
  • Target See all BDNF Proteins
    BDNF (Brain-Derived Neurotrophic Factor (BDNF))
    Protein Type
    Recombinant
    Origin
    • 19
    • 8
    • 6
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Source
    • 30
    • 17
    • 3
    • 2
    • 1
    Escherichia coli (E. coli)
    Application
    SDS-PAGE (SDS)
    Supplier Product No.
    009-001-c27
    Supplier
    Rockland
    Purpose
    BDNF Human Recombinant Protein
    Purification
    Purity was determined to be greater than 97% as determined by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE against a known standard. Endotoxin level as determined by kinetic LAL analysis: 0.05 EUs/μg protein.
    Purity
    97,00%
    Endotoxin Level
    Measured by LAL is < 0.01 ng/μg or < 0.1 EU/μg protein.
    Biological Activity Comment
    Proliferation of a neuroblastoma cell line stably expressing TrkB (BR6) ED50 ≤ 40 ng/mL (≥ 2.5 x 10^4 units/mg).
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    Discover our top product BDNF Protein
  • Application Notes
    BDNF has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-BDNF in immunological assays.

    Comment

    Suggested_Applications: Other

    Other_Performance_Data:

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution_Buffer: Restore with deionized water (or equivalent)

    Reconstitution_Volume: 10 μL (10-100 μL)

    Concentration
    0.1 mg/mL
    Buffer

    Buffer: 0.1 % Trifluoroacetic acid

    Stabilizer: None

    Preservative
    Without preservative
    Storage
    -20 °C
    Storage Comment
    Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
    Expiry Date
    6 months
  • Chowdhury, Collins, Gell, Perry, Breadmore, Shigdar, King: "Isolation and Identification of the High-Affinity DNA Aptamer Target to the Brain-Derived Neurotrophic Factor (BDNF)." in: ACS chemical neuroscience, Vol. 15, Issue 2, pp. 346-356, (2024) (PubMed).

  • Target
    BDNF (Brain-Derived Neurotrophic Factor (BDNF))
    Alternative Name
    BDNF (BDNF Products)
    Background

    Synonyms: Brain-derived neurotrophic factor, Abrineurin cytokine

    Background: BDNF is a member of the nerve growth factor family of trophic factors. In the brain BDNF has a trophic action on retinal, cholinergic, and dopaminergic neurons, and in the peripheral nervous system it acts on both motor and sensory neurons. Some protein domains of BDNF are identical with those of NGF and another neurotrophic factor, designated NT-3 (neurotrophin-3). It exists as monomers and homodimers, and binds to NTRK2/TRKB. Polyclonal antibodies raised against murine NGF have been shown to cross-react with both NT-3 and BDNF. The propeptide is N-glycosylated and glycosulfated. BDNF is converted into mature BDNF by plasmin (PLG). It is expressed in brain, and highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum, it is also expressed in heart, lung, skeletal muscle, testis, prostate and placenta. Recombinant Human Brain-Derived Neurotrophic Factor produced in E.coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 119 amino acids and having a total molecular mass of 26,984 Daltons.

    UniProt
    P23560
    Pathways
    RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity
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