ARTN Protein
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- Target See all ARTN Proteins
- ARTN (Artemin (ARTN))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Supplier Product No.
- 009-001-u64-0005
- Supplier
- Rockland
- Purpose
- Human Artemin Recombinant Protein
- Purification
- Artemin purity was determined to be greater than 98% as determined by analysis by HpLC and by reducing and non-reducing SDS-pAGE.
- Purity
- 98,00%
- Endotoxin Level
- Measured by LAL is typically ≤ 1 EU/μg protein.
- Biological Activity Comment
- The activity is determined by the dose-dependant proliferation of the SH-SY5Y cell line and is typically 4-8 ng/mL. The activity can also be determined by its ability to promote survival and neurite outgrowth.
- Top Product
- Discover our top product ARTN Protein
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Artemin (ARTN) (AA 112-224) protein (Fc Tag)
ARTN Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Artemin (ARTN) (AA 108-220) protein (Fc Tag)ARTN Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Artemin (ARTN) (AA 108-220) protein (His tag)ARTN Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Artemin (ARTN) (AA 1-228) protein (GST tag)ARTN Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Artemin (ARTN) proteinARTN Origin: Human Host: Escherichia coli (E. coli) Recombinant 98,00% SDS
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- Application Notes
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Other: User Optimized
Application_Note: Recombinant human Artemin has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-Artemin in immunological assays.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 5 μL (5-50 μL)
- Buffer
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Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
- Preservative
- Without preservative
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- Expiry Date
- 6 months
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- Target
- ARTN (Artemin (ARTN))
- Alternative Name
- ARTN (ARTN Products)
- Background
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Synonyms: ARTN, enovin, neublastin
Background: Artemin is a novel member of the glial cell line-derived neurotrophic factor (GNDF) ligand family. Current evidence suggests that Artemin signals through the receptor complex, GFRα3-RET, to influence neuron survival in vitro and in vivo. Recombinant human Artemin is a non-glycosylated, disulfide-linked homodimer, containing two 113 amino acid chains, with a total molecular weight of 24.2 kDa.
- UniProt
- Q5T4W7
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