Luciferase Protein (AA 1-550) (His tag)
Origin: Firefly
Host: Escherichia coli (E. coli)
Recombinant
> 90 by SDS - PAGE
SDS
1 image
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- Target See all Luciferase Proteins
- Luciferase
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-550
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Origin
- Firefly
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Luciferase protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Characteristics
- Luciferase, 1-550aa, Firefly, His tag, E.coli
- Purity
- > 90 by SDS - PAGE
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Luciferase (AA 1-311) (Active) protein (His tag)
Origin: Sea Pansy Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE SDS Active
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1mg/ml (determined by Bradford assay)
- Buffer
- Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
- Storage
- 4 °C
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- Target
- Luciferase
- Abstract
- Luciferase Products
- Synonyms
- hypothetical protein Protein, luciferase Protein, C25G6.3 Protein, Gbro_2186 Protein
- Background
- Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP, produces green light with a wavelength of 562 nm. Luciferase from firefly is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. Recombinant firefly Luciferase, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Luciferin 4-monooxygenase, LUC. NCBI no.: BAF48390
- Molecular Weight
- 62.9 kDa (571aa), confirmed by MALDI-TOF
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