DnaK (AA 1-384) Protein

Catalog No. ABIN666809

Product Details

Target: DnaK

Protein Type: Recombinant

Protein Characteristics: AA 1-384

Origin: E. coli

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Characteristics: Dnak(N-term, 1-384), E.coli, Recombinant, E.coli)

Purity: > 95 % by SDS - PAGE

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/ml (determined by Bradford assay)

Buffer: Liquid. 25 mM Tris-HCl, pH7.5, 100 mM NaCl, 5 mM DTT, 10%Glycerol.

Storage: 4 °C

Target Details

Target: DnaK

Background: DnaK, originally identified for its DNA replication by bacteriophage lambda in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. DnaK(amino acids1-384) is N-terminal ATPase domain and ATP bound to the ATPase domain induces a conformational change in the substrate binding domain(residues385-638). The protein coding region of the ATPase domain of DNAK (amino acids 1-384) was amplified by PCR and cloned into an E. coli expression vector. The ATPase domain of DNAK was overexpressed in E. coli and the recombinant protein was purified to apparent homogeneity by using conventional column chromatography techniques. Synonyms: dnaK, Dnak (N-term, 1-384), ATPase binding domain, Heat shock protein 70, Heat shock 70 kDa protein, HSP70, Chaperone protein dnaK, Chaperone Hsp70, Co chaperone with DnaJ,. NCBI no.: NP_414555

Molecular Weight: 41.6kDa (384aa)