DnaK (AA 1-638), (full length) Protein

Catalog No. ABIN666804

Product Details

Target: DnaK

Protein Type: Recombinant

Protein Characteristics: full length, AA 1-638

Origin: E. coli

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Characteristics: Dnak(full length, 1-638) E.coli, Recombinant, E.coli

Purity: > 95 % by SDS - PAGE

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/ml (determined by Bradford assay)

Buffer: Liquid. 25 mM Tris-HCl, pH7.5, 100 mM NaCl, 5mM DTT,10%Glycerol

Storage: 4 °C

Target Details

Target: DnaK

Background: DnaK, originally identified for its DNA replication by bacteriophage lambda in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. DnaK (amino acids 1-638) was amplified by PCR and cloned into an E. coli expression vector. DnaK 1-638 was overexpressed in E. coli and was purified to apparent homogeneity by using conventional column chromatography techniques. Synonyms: Chaperone protein dnaK, HSP70, groP, grpF, seg, Heat shock protein 70, Chaperone Hsp70, Co chaperone with DnaJ, dnaK, Heat shock 70 kDa protein,. NCBI no.: NP_414555

Molecular Weight: 69kDa (638aa)