CDC42 Protein (AA 1-188) (T7 tag)
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- Target See all CDC42 Proteins
- CDC42 (Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-188
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This CDC42 protein is labelled with T7 tag.
- Application
- SDS-PAGE (SDS)
- Characteristics
- CDC42, 1- 188 aa, Human, T7-tagged, Recombinant, E.coli
- Purity
- > 95 % by SDS - PAGE
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol, 2mM EDTA
- Storage
- 4 °C
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- Target
- CDC42 (Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42))
- Alternative Name
- CDC42 (CDC42 Products)
- Synonyms
- CDC42Hs Protein, G25K Protein, AI747189 Protein, AU018915 Protein, cdc42a Protein, wu:fb07d08 Protein, wu:fj62g06 Protein, zgc:63918 Protein, Xcdc42 Protein, cdc42hs Protein, g25k Protein, CDC42 Protein, CG12530 Protein, Cdc42Dm Protein, D-CDC42 Protein, D-Cdc42 Protein, D-cdc42 Protein, DCDC42 Protein, DCdc42 Protein, Dcdc42 Protein, Dm Cdc42 Protein, DmCDC42 Protein, Dmcdc42 Protein, Dmel\\CG12530 Protein, cdc42 Protein, cell division cycle 42 Protein, Cell division control protein 42 homolog Protein, cell division control protein 42 homolog Protein, cell division cycle 42 L homeolog Protein, CG12530 gene product from transcript CG12530-RC Protein, Rho family GTPase Protein, CDC42 Protein, Cdc42 Protein, cdc42 Protein, LOC100636735 Protein, LOC100637699 Protein, LOC100638834 Protein, cdc42.L Protein
- Background
- Cell division cycle 42 isoform 1, also known as CDC42, is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. Also, this protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Loss of CDC42 function caused an increase in the endocytotic uptake of apical proteins, including apical polarity factors such as Crumbs. Recombinant human CDC42, fused to T7-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: G25K, Cell division cycle 42 isoform 1 CDC42Hs, Cell division cycle 42, Small GTP binding protein CDC42. NCBI no.: NP_001782
- Molecular Weight
- 22.4 kDa (203aa), confirmed by MALDI-TOF.
- Pathways
- MAPK Signaling, Microtubule Dynamics, RTK Signaling, WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Maintenance of Protein Location, Skeletal Muscle Fiber Development, Signaling Events mediated by VEGFR1 and VEGFR2, EGFR Downregulation, VEGF Signaling
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