MEK1 Protein (AA 1-393) (His tag)

Catalog No. ABIN6388205

Product Details

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Protein Type: Recombinant

Protein Characteristics: AA 1-393

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This MEK1 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: ADPMPKKKPT PIQLNPAPDG SAVNGTSSAE TNLEALQKKL EELELDEQQR KRLEAFLTQK QKVGELKDDD FEKISELGAG NGGVVFKVSH KPSGLVMARK LIHLEIKPAI RNQIIRELQV LHECNSPYIV GFYGAFYSDG EISICMEHMD GGSLDQVLKK AGRIPEQILG KVSIAVIKGL TYLREKHKIM HRDVKPSNIL VNSRGEIKLC DFGVSGQLID SMANSFVGTR SYMSPERLQG THYSVQSDIW SMGLSLVEMA VGRYPIPPPD AKELELMFGC QVEGDAAETP PRPRTPGRPL SSYGMDSRPP MAIFELLDYI VNEPPPKLPS GVFSLEFQDF VNKCLIKNPA ERADLKQLMV HAFIKRSDAE EVDFAGWLCS TIGLNQPSTP THAAGVHHHH HH

Purity: > 90 % by SDS - PAGE

Endotoxin Level: < 1.0 EU per 1ug of protein (determined by LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Alternative Name: MAP2K1 (MAP2K1 Products)

Synonyms: Mek1 Protein, CFC3 Protein, MAPKK1 Protein, MEK1 Protein, MKK1 Protein, PRKMK1 Protein, MEKK1 Protein, Prkmk1 Protein, mek-2 Protein, si:ch211-242m18.2 Protein, wu:fj56a12 Protein, wu:fj61b01 Protein, zgc:56557 Protein, ATMEK1 Protein, F20B18.180 Protein, F20B18_180 Protein, MAP kinase/ ERK kinase 1 Protein, MITOGEN ACTIVATED PROTEIN KINASE KINASE Protein, MITOGEN ACTIVATED PROTEIN KINASE KINASE 1 Protein, NMAPKK Protein, MEK Protein, mitogen activated protein kinase kinase 1 Protein, mitogen-activated protein kinase kinase 1 Protein, mitogen-activated protein kinase kinase 1 L homeolog Protein, MAP kinase/ ERK kinase 1 Protein, Map2k1 Protein, MAP2K1 Protein, map2k1.L Protein, map2k1 Protein, MEK1 Protein

Background: MAP2K1, is a member of the bispecific protein kinase family, which acts as a mitogen-activated protein (MAP) kinase. It is expressed extensively and brain levels are very low. It is located upstream of the MAP kinase and stimulates the enzymatic activity of MAP kinases on a variety of extra-and-cellular signals. This protein, an essential component of the MAP kinase signaling pathway, is involved in many cellular processes such as proliferation, differentiation, transcriptional regulation and development. Recombinant human MAP2K1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molecular Weight: 44.5kDa (402aa) 40-57kDa (SDS-PAGE under reducing conditions)

NCBI Accession: NP_002746

UniProt: Q02750

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling