DPH2 Protein (AA 1-489) (His tag)

Catalog No. ABIN6388024

Product Details

Target: DPH2 (Diphthamide Biosynthesis Protein 2 (DPH2))

Protein Type: Recombinant

Protein Characteristics: AA 1-489

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This DPH2 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MESMFSSPAE AALQRETGVP GLLTPLPDLD GVYELERVAG FVRDLGCERV ALQFPDQLLG DAVAVAARLE ETTGSKMFIL GDTAYGSCCV DVLGAEQAGA QALIHFGPAC LSPPARPLPV AFVLRQRSVA LELCVKAFEA QNPDPKAPVV LLSEPACAHA LEALATLLRP RYLDLLVSSP AFPQPVGSLS PEPMPLERFG RRFPLAPGRR LEEYGAFYVG GSKASPDPDL DPDLSRLLLG WAPGQPFSSC CPDTGKTQDE GARAGRLRAR RRYLVERARD ARVVGLLAGT LGVAQHREAL AHLRNLTQAA GKRSYVLALG RPTPAKLANF PEVDVFVLLA CPLGALAPQL SGSFFQPILA PCELEAACNP AWPPPGLAPH LTHYADLLPG SPFHVALPPP ESELWETPDV SLITGDLRPP PAWKSSNDHG SLALTPRPQL ELAESSPAAS FLSSRSWQGL EPRLGQTPVT EAVSGRRGIA IAYEDEGSGL EHHHHHH

Purity: > 85 % by SDS - PAGE

Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 1  mM DTT, 20 % glycerol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: DPH2 (Diphthamide Biosynthesis Protein 2 (DPH2))

Alternative Name: DPH2 (DPH2 Products)

Synonyms: DPH2L2 Protein, 9130020C19Rik Protein, AI467389 Protein, Dph2l2 Protein, id:ibd5058 Protein, zgc:162269 Protein, DPH2 homolog Protein, DPH2 homolog (S. cerevisiae) Protein, hypothetical protein Protein, DPH2 Protein, Dph2 Protein, dph2 Protein, CAALFM_C400690CA Protein

Background: DPH2, also known as diphthamide biosynthesis protein 2, is a homodimer and each of its monomers can bind a [4Fe-4S] cluster. It is the target of ADP ribosylating diphtheria toxin (DT) and Pseudomonas exotoxin A (PE). It was identified by its ability to complement a diphthamide mutant strain, and thus functions in diphthamide biosynthesis. Its loss pre-activates NF-kB and death receptor pathways and renders MCF7 cells hypersensitive to tumor necrosis factor. Recombinant human DPH2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molecular Weight: 53.1kDa (497aa) 50-70kDa (SDS-PAGE under reducing conditions)

NCBI Accession: NP_001375

UniProt: Q9BQC3