DKK3 Protein (AA 22-350) (His tag)
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- Target See all DKK3 products
- DKK3
- Protein Type
- Recombinant
- Protein Characteristics
- AA 22-350
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This DKK3 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- ADPAPAPTAT SAPVKPGPAL SYPQEEATLN EMFREVEELM EDTQHKLRSA VEEMEAEEAA AKASSEVNLA NLPPSYHNET NTDTKVGNNT IHVHREIHKI TNNQTGQMVF SETVITSVGD EEGRRSHECI IDEDCGPSMY CQFASFQYTC QPCRGQRMLC TRDSECCGDQ LCVWGHCTKM ATRGSNGTIC DNQRDCQPGL CCAFQRGLLF PVCTPLPVEG ELCHDPASRL LDLITWELEP DGALDRCPCA SGLLCQPHSH SLVYVCKPTF VGSRDQDGEI LLPREVPDEY EVGSFMEEVR QELEDLERSL TEEMALREPA AAAAALLGGE EIHHHHHH
- Purity
- > 90 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
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DKK3 (AA 23-350) protein (His-Avi Tag)
Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
DKK3 (AA 23-350) protein (His-Avi Tag,Biotin)Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
DKK3 (AA 1-350) protein (GST tag)Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- DKK3
- Abstract
- DKK3 Products
- Synonyms
- REIC Protein, RIG Protein, reic Protein, dkk-3 Protein, AW061014 Protein, C87148 Protein, Reic Protein, p29 Protein, dickkopf WNT signaling pathway inhibitor 3 Protein, dickkopf WNT signaling pathway inhibitor 3 S homeolog Protein, DKK3 Protein, dkk3 Protein, dkk3.S Protein, Dkk3 Protein
- Background
- DKK3, also known as dickkopf WNT signaling pathway inhibitor 3, is a member of the DKK protein family including Dkk-1, 2, 3 and 4. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Recombinant human DKK3 protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 37.3kDa (338aa) 40-57KDa (SDS-PAGE under reducing conditions.)
- NCBI Accession
- NP_001018067
- UniProt
- Q9UBP4
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