PLAT Protein (AA 311-562) (His tag)
-
- Target See all PLAT Proteins
- PLAT (Plasminogen Activator, Tissue (PLAT))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 311-562
-
Origin
- Human
-
Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This PLAT protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- QEIHARFRRG ARSYQVICRD EKTQMIYQQH QSWLRPVLRS NRVEYCWCNS GRAQCHSVPV KSCSEPRCFN GGTCQQALYF SDFVCQCPEG FAGKCCEIDT RATCYEDQGI SYRGTWSTAE SGAECTNWNS SALAQKPYSG RRPDAIRLGL GNHNYCRNPD RDSKPWCYVF KAGKYSSEFC STPACSEGNS DCYFGNGSAY RGTHSLTESG ASCLPWNSMI LIGKVYTAQN PSAQALGLGK HNYCRNPDGD AKPWCHVLKN RRLTWEYCDV PSCSTCGLRQ YSQPQFRIKG GLFADIASHP WQAAIFAKHR RSPGERFLCG GILISSCWIL SAAHCFQERF PPHHLTVILG RTYRVVPGEE EQKFEVEKYI VHKEFDDDTY DNDIALLQLK SDSSRCAQES SVVRTVCLPP ADLQLPDWTE CELSGYGKHE ALSPFYSERL KEAHVRLYPS SRCTSQHLLN RTVTDNMLCA GDTRSGGPQA NLHDACQGDS GGPLVCLNDG RMTLVGIISW GLGCGQKDVP GVYTKVTNYL DWIRDNMRPH HHHHH
- Purity
- > 90 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Top Product
- Discover our top product PLAT Protein
-
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In 50 mM MES buffer ( pH 5.5) containing 40 % glycerol, 5 mM CaCl2, 1 mM DTT, 0.5M NaCl.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
-
- Target
- PLAT (Plasminogen Activator, Tissue (PLAT))
- Alternative Name
- PLAT (PLAT Products)
- Synonyms
- T-PA Protein, TPA Protein, AU020998 Protein, AW212668 Protein, D8Ertd2e Protein, tPA Protein, PATISS Protein, tpa Protein, Plat Protein, plat Protein, t-pa Protein, plasminogen activator, tissue type Protein, plasminogen activator, tissue Protein, chromosome 20 open reading frame 181 Protein, plasminogen activator, tissue L homeolog Protein, PLAT Protein, Plat Protein, plat Protein, C20orf181 Protein, plat.L Protein
- Background
- PLAT, as known as tissue-type plasminogen activator isoform 1, is a secreted serine protease synthesized by endothelial cells. This protein is secreted as a single chain polypeptide precursor which is cleaved in turn by plasmin. Also, it plays a role clinical medicine to treat stroke in cell migration and tissue remodeling. It is implicated in normal neural function and is suggested as a regulatory molecule in neurodegenerative diseases. Recombinant human PLAT, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 61.3kDa (545aa) 50-70kDa (SDS-PAGE under reducing conditions.)
- NCBI Accession
- NP_000921
- UniProt
- P00750
- Pathways
- Autophagy, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, SARS-CoV-2 Protein Interactome
-