AKR1C4 Protein (AA 1-323)
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- Target See all AKR1C4 Proteins
- AKR1C4 (Aldo-keto Reductase Family 1 Member C4 (AKR1C4))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-323
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Sequence
- MDPKYQRVEL NDGHFMPVLG FGTYAPPEVP RNRAVEVTKL AIEAGFRHID SAYLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCTFF QPQMVQPALE SSLKKLQLDY VDLYLLHFPM ALKPGETPLP KDENGKVIFD TVDLSATWEV MEKCKDAGLA KSIGVSNFNC RQLEMILNKP GLKYKPVCNQ VECHPYLNQS KLLDFCKSKD IVLVAHSALG TQRHKLWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIREN IQVFEFQLTS EDMKVLDGLN RNYRYVVMDF LMDHPDYPFS DEY
- Purity
- > 90 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1ug of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is > 700 pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C.
- Top Product
- Discover our top product AKR1C4 Protein
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Aldo-keto Reductase Family 1 Member C4 (AKR1C4) (AA 1-343) protein (His tag)
AKR1C4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Aldo-keto Reductase Family 1 Member C4 (AKR1C4) (AA 1-323) protein (GST tag)AKR1C4 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Aldo-keto Reductase Family 1 Member C4 (AKR1C4) protein (Myc-DYKDDDDK Tag)AKR1C4 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer( pH 8.5) containing 0.1M NaCl, 10 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- AKR1C4 (Aldo-keto Reductase Family 1 Member C4 (AKR1C4))
- Alternative Name
- Aldo-keto reductase family 1 member C4 (AKR1C4 Products)
- Synonyms
- 3-alpha-HSD Protein, C11 Protein, CDR Protein, CHDR Protein, DD-4 Protein, DD4 Protein, HAKRA Protein, aldo-keto reductase family 1 member C4 Protein, AKR1C4 Protein
- Background
- AKR1C1 also known as Aldo-keto reductase family 1 member C4, belongs to aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. It catalyzes the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. Recombinant human AKR1C4 protein was expressed in E.coli and purified by using conventional chromatography.
- Molecular Weight
- 37kDa (323aa)
- NCBI Accession
- NP_001809
- UniProt
- P17516
- Pathways
- Steroid Hormone Biosynthesis
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