AKR1C3 Protein (AA 1-323, Isoform 1)
-
- Target See all AKR1C3 Proteins
- AKR1C3 (Aldo-Keto Reductase Family 1, Member C3 (3-alpha Hydroxysteroid Dehydrogenase, Type II) (AKR1C3))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-323, Isoform 1
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Sequence
- MDSKHQCVKL NDGHFMPVLG FGTYAPPEVP RSKALEVTKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWSTFH RPELVRPALE NSLKKAQLDY VDLYLIHSPM SLKPGEELSP TDENGKVIFD IVDLCTTWEA MEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNRS KLLDFCKSKD IVLVAYSALG SQRDKRWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTA EDMKAIDGLD RNLHYFNSDS FASHPNYPYS DEY
- Purity
- > 90 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1ug of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is > 1,000 pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C.
- Top Product
- Discover our top product AKR1C3 Protein
-
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
Bioactivity Validated
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.5) containing 0.1M NaCl, 10 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
-
- Target
- AKR1C3 (Aldo-Keto Reductase Family 1, Member C3 (3-alpha Hydroxysteroid Dehydrogenase, Type II) (AKR1C3))
- Alternative Name
- Aldo-keto reductase family 1 member C3 (AKR1C3 Products)
- Synonyms
- AKR1C3 Protein, PGFS Protein, MGC134333 Protein, DD3 Protein, DDX Protein, HA1753 Protein, HAKRB Protein, HAKRe Protein, HSD17B5 Protein, hluPGFS Protein, Akr1c18 Protein, aldo-keto reductase family 1, member C3 L homeolog Protein, aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II) Protein, aldo-keto reductase family 1 member C2 Protein, aldo-keto reductase family 1 member C3 Protein, aldo-keto reductase family 1, member C3 Protein, akr1c3.L Protein, AKR1C3 Protein, LOC722496 Protein, Akr1c3 Protein
- Background
- AKR1C3 also known as Aldo-keto reductase family 1 member C3 isoform 1, is a member of the aldo-keto reductase superfamily which catalyzes the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9 alpha, 11 beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and differentiation. Recombinant human AKR1C3 protein was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 36.8kDa (323aa)
- NCBI Accession
- NP_003730
- UniProt
- P42330
- Pathways
- Retinoic Acid Receptor Signaling Pathway, Steroid Hormone Biosynthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, C21-Steroid Hormone Metabolic Process, Protein targeting to Nucleus
-