NMNAT1 Protein (AA 115-270) (His tag)
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- Target See all NMNAT1 Proteins
- NMNAT1 (Nicotinamide Nucleotide Adenylyltransferase 1 (NMNAT1))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 115-270
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NMNAT1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMENS EKTEVVLLAC GSFNPITNMH LRLFELAKDY MNGTGRYTVV KGIISPVGDA YKKKGLIPAY HRVIMAELAT KNSKWVEVDT WESLQKEWKE TLKVLRHHQE KLEASDCDHQ QNSPTLERPG RKRKWTETQD SSQKKSLEPK TKAVPKVKLL CGADLLESFA VPNLWKSEDI TQIVANYGLI CVTRAGNDAQ KFIYESDVLW KHRSNIHVVN EWIANDISST KIRRALRRGQ SIRYLVPDLV QEYIEKHNLY SSESEDRNAG VILAPLQRNT AEAK
- Purity
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 7,000 pmol/min/ug, and was obtained by measuring the beta-NAD from nicotinamide mononucleotide and ATP per minute at pH 8.0 at 37C.
- Top Product
- Discover our top product NMNAT1 Protein
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Nicotinamide Nucleotide Adenylyltransferase 1 (NMNAT1) (AA 1-279) protein (His tag)
NMNAT1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Nicotinamide Nucleotide Adenylyltransferase 1 (NMNAT1) (AA 1-285) protein (His tag)NMNAT1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Nicotinamide Nucleotide Adenylyltransferase 1 (NMNAT1) (AA 1-279) protein (GST tag)NMNAT1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Nicotinamide Nucleotide Adenylyltransferase 1 (NMNAT1) (AA 51-150) protein (GST tag)NMNAT1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Nicotinamide Nucleotide Adenylyltransferase 1 (NMNAT1) protein (Myc-DYKDDDDK Tag)NMNAT1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol, 0.1M NaCl, 1 mM DTT. 1 mM EDTA
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- NMNAT1 (Nicotinamide Nucleotide Adenylyltransferase 1 (NMNAT1))
- Alternative Name
- NMNAT1 (NMNAT1 Products)
- Synonyms
- id:ibd5068 Protein, im:7144541 Protein, zgc:110243 Protein, LCA9 Protein, NMNAT Protein, PNAT1 Protein, 2610529L11Rik Protein, 5730441G13Rik Protein, D4Cole1e Protein, nmnat Protein, nicotinamide mononucleotide adenylyltransferase 1 Protein, nicotinamide nucleotide adenylyltransferase 1 Protein, CpipJ_CPIJ015320 Protein, PTRG_06486 Protein, nmnat1 Protein, NMNAT1 Protein, Nmnat1 Protein
- Background
- NMNAT1, also known as NMNAT or PNAT1, is a central enzyme in NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD. It is widely expressed with high levels in skeletal muscle, heart, liver and kidney. This protein appears to have the ability to protect against axonal degeneration following mechanical or toxic insults. Recombinant human NMNAT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 36.0 kDa (315aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_073624
- UniProt
- Q9HAN9
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