GLO1 Protein (AA 1-184)
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- Target See all GLO1 Proteins
- GLO1 (Glyoxalase I (GLO1))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-184
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Sequence
- ARPCIPKSFG YSSVVCVCNA TYCDSFDPPT FPALGTFSRY ESTRSGRRME LSMGPIQANH TGTGLLLTLQ PEQKFQKVKG FGGAMTDAAA LNILALSPPA QNLLLKSYFS EEGIGYNIIR VPMASCDFSI RTYTYADTPD DFQLHNFSLP EEDTKLKIPL IHRALQLAQR PVSLLASPWT SPTWLKTNGA VNGKGSLKGQ PGDIYHQTWA RYFVKFLDAY AEHKLQFWAV TAENEPSAGL LSGYPFQCLG FTPEHQRDFI ARDLGPTLAN STHHNVRLLM LDDQRLLLPH WAKVVLTDPE AAKYVHGIAV HWYLDFLAPA KATLGETHRL FPNTMLFASE ACVGSKFWEQ SVRLGSWDRG MQYSHSIITN LLYHVVGWTD WNLALNPEGG PNWVRNFVDS PIIVDITKDT FYKQPMFYHL GHFSKFIPEG SQRVGLVASQ KNDLDAVALM HPDGSAVVVV LNRSSKDVPL TIKDPAVGFL ETISPGYSIH TYLWRRQHHH HHH
- Purity
- > 90 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity: > 400 units/mg. One unit will form 1.0umol of S-lactoylgutathione from methylglyoxal and reduced glutathione per minute at pH6.5 at 25C.
- Top Product
- Discover our top product GLO1 Protein
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Glyoxalase I (GLO1) (AA 2-184) protein (His tag)
GLO1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Glyoxalase I (GLO1) (AA 1-184) proteinGLO1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS
Glyoxalase I (GLO1) (AA 1-184) protein (GST tag)GLO1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Glyoxalase I (GLO1) protein (His tag)GLO1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE
Glyoxalase I (GLO1) (AA 1-184) (Active) protein (His tag)GLO1 Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 95 % by SDS - PAGE SDS Active
Glyoxalase I (GLO1) protein (Myc-DYKDDDDK Tag)GLO1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- GLO1 (Glyoxalase I (GLO1))
- Alternative Name
- GLO1 (GLO1 Products)
- Background
- Lactoylglutathione lyase, also known as GLO1, belongs to the glyoxalaseIfamily. Glyoxalase I is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutathione.This enzyme is ubiquitously expressed and is also present in many tumor cell lines, in which its concentration is often upregulated. Recombinant human GLO1 protein was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 20.7 kDa (184 aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_006699
- UniProt
- Q04760
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