GALT Protein (His tag)

Catalog No. ABIN6387110

Product Details

Target: GALT (Galactose-1-Phosphate Uridylyltransferase (GALT))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This GALT protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Purification: purified by chromatography

Purity: > 85 % by SDS - PAGE

Application Details

Comment:

Synonyms: Galactose-1-phosphate uridylyltransferase, Gal-1-P uridylyltransferase, UDP-glucose--hexose-1-phosphate uridylyltransferase

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.5) containing 0.2 M NaCl, 10% glycerol.

Storage: 4 °C

Storage Comment: Avoid repeated freezing and thawing cycles.

Target Details

Target: GALT (Galactose-1-Phosphate Uridylyltransferase (GALT))

Alternative Name: GALT (GALT Products)

Synonyms: GALT Protein, AW553376 Protein, CG9232 Protein, Dmel\\CG9232 Protein, dGALT Protein, galactose-1-phosphate uridylyltransferase Protein, galactose-1-phosphate uridylyltransferase, GalT Protein, probable galactose-1-phosphate uridylyltransferase galtb [second part] Protein, UDP-glucose--galactose-1-phosphate uridylyltransferase Protein, GalT galactose-1-phosphate uridylyltransferase Protein, galactose-1-phosphate uridyl transferase Protein, Galactose-1-phosphate uridylyltransferase Protein, galactose-1-phosphate uridylyltransferase S homeolog Protein, GALT Protein, galT Protein, Galt Protein, CNM00620 Protein, Mrub_2813 Protein, Mesil_2395 Protein, Trad_0989 Protein, Igag_1846 Protein, VDIS_RS10425 Protein, Intca_2810 Protein, Marky_2157 Protein, Selsp_1876 Protein, Trebr_2368 Protein, Halhy_5538 Protein, Theth_1357 Protein, galt.S Protein

Background: GALT catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human GALT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques

Molecular Weight: 45.9 kDa (403aa), confirmed by MALDI-TOF

NCBI Accession: NP_000146