GALT Protein (His tag)
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- Target See all GALT Proteins
- GALT (Galactose-1-Phosphate Uridylyltransferase (GALT))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This GALT protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product GALT Protein
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Galactose-1-Phosphate Uridylyltransferase (GALT) (AA 1-379) protein (GST tag)
GALT Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Galactose-1-Phosphate Uridylyltransferase (GALT) proteinGALT Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % pure SDS
Galactose-1-Phosphate Uridylyltransferase (GALT) (AA 1-379), (full length) protein (His-SUMO Tag)GALT Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Galactose-1-Phosphate Uridylyltransferase (GALT) protein (Myc-DYKDDDDK Tag)GALT Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Comment
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Synonyms: Galactose-1-phosphate uridylyltransferase, Gal-1-P uridylyltransferase, UDP-glucose--hexose-1-phosphate uridylyltransferase
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.5) containing 0.2 M NaCl, 10% glycerol.
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- GALT (Galactose-1-Phosphate Uridylyltransferase (GALT))
- Alternative Name
- GALT (GALT Products)
- Background
- GALT catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human GALT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques
- Molecular Weight
- 45.9 kDa (403aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_000146
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