Flagellin Protein (FliC) (Mutant) (His tag)
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- Target See all Flagellin (FliC) Proteins
- Flagellin (FliC)
- Protein Type
- Recombinant
- Protein Characteristics
- Mutant
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Origin
- Human, Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Flagellin protein is labelled with His tag.
- Purpose
- Flagellin (TLR5 Mutant) (rec.)
- Specificity
- The native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) contains a substitution of amino acids 90-97 required for TLR5 signaling by a motif from a non-signaling flagellin and is fused at the N-terminus to a His-tag.
- Cross-Reactivity
- Human, Mouse
- Characteristics
- The native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) contains a substitution of amino acids 90-97 required for TLR5 signaling by a motif from a non-signaling flagellin and is fused at the N-terminus to a His-tag.
- Purity
- >95 % (SDS-PAGE)
- Endotoxin Level
- <0.01EU/μg purified protein (LAL test).
- Biological Activity Comment
- Does not activate TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, in vivo on expression of CCL20 or IL-6. It can be used as negative control for TLR5 signaling assays.
- Top Product
- Discover our top product FliC Protein
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Flagellin (FliC) (AA 2-498) protein (His tag)
FliC Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Flagellin (FliC) proteinFliC Origin: Borrelia burgdorferi Host: Escherichia coli (E. coli) Recombinant ELISA, WB
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- 0.1 mg/mL
- Buffer
- Contains PBS.
- Handling Advice
- After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 6 months after receipt when stored at -20°C.
- Expiry Date
- 6 months
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- Target
- Flagellin (FliC)
- Alternative Name
- Flagellin (FliC Products)
- Background
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FliC (TLR5 Mutant)
Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) or the intracellular NLRC4 protein. The Flagellin (TLR5 Mutant) (rec.) cannot be detected by human and mouse TLR5. It has not been tested on NLRC4.
- Pathways
- Inflammasome
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