DNASE1 Protein (AA 19-259) (His tag)
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- Target See all DNASE1 Proteins
- DNASE1 (Deoxyribonuclease I (DNASE1))
- Biological Activity
- Active
- Protein Characteristics
- AA 19-259
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This DNASE1 protein is labelled with His tag.
- Application
- Cell Culture (CC), Activity Assay (AcA)
- Characteristics
- Tag location: N-terminal His Tag
- Purity
- > 90 %
- Biological Activity Comment
- Deoxyribonuclease I (usually called DNase I) is a nonspecific endonuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. It acts on single-stranded DNA, double-stranded DNA, and chromatin. DNase I can be activated by bivalent metals such as Mg2+ and Ca2+. This endonuclease enzyme is common reagents used in biochemical methods requiring diestion of DNA and recovery of RNA, or where DNA is to be removed without affecting structural proteins or enzymes. For example, DNase I is frequently used to remove template DNA following in vitro transcription, and to remove contaminating DNA in total RNA preparations (especially those from transfected cells that may contain plasmid DNA), used for ribonuclease protection assays, cDNA library contraction, and RT-PCR. Besides, Actin Beta (ACTb) has been identified as an interactor of DNase I, thus a binding ELISA assay was conducted to detect the interaction of recombinant human DNase I and recombinant human ACTb. Briefly, DNase I were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ACTb-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-DNase I pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of DNase I and ACTb was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of DNASE I with ACTb.
- Top Product
- Discover our top product DNASE1 Protein
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- Application Notes
- Isoelectric Point: 5
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
- Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Target
- DNASE1 (Deoxyribonuclease I (DNASE1))
- Abstract
- DNASE1 Products
- Synonyms
- LOC397802 Protein, DNL1 Protein, DRNI Protein, AI788650 Protein, DNaseI Protein, Dnl1 Protein, dnase1-A Protein, zgc:92440 Protein, DNase I Protein, deoxyribonuclease 1 Protein, deoxyribonuclease I Protein, deoxyribonuclease I L homeolog Protein, Deoxyribonuclease I Protein, endA Protein, LOC397802 Protein, DNASE1 Protein, Dnase1 Protein, dnase1.L Protein, dnase1 Protein, CpipJ_CPIJ011184 Protein, CpipJ_CPIJ011185 Protein, CpipJ_CPIJ011186 Protein, CpipJ_CPIJ011187 Protein, CpipJ_CPIJ011190 Protein, CpipJ_CPIJ017803 Protein, Desal_1409 Protein, MPET_RS05255 Protein, METEV_RS02275 Protein, Plabr_0631 Protein
- Background
- Alternative Names: DNaseI, DNL1, DRNI, DNase-I, Dornase alfa
- Molecular Weight
- 28kDa
- UniProt
- P24855
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