ALDH1A1 Protein (AA 2-501) (His tag)
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- Target See all ALDH1A1 Proteins
- ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))
- Biological Activity
- Active
- Protein Characteristics
- AA 2-501
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This ALDH1A1 protein is labelled with His tag.
- Application
- Cell Culture (CC), Activity Assay (AcA)
- Characteristics
- Tag location: N-terminal His Tag
- Purity
- > 95 %
- Biological Activity Comment
- ALDH1A1 (Retinal dehydrogenase 1) which belongs to the aldehyde dehydrogenase family, is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. ALDH1A1 converts retinaldehyde to retinoic acid. IL21 (interleukin 21) was identified as an interactor of ALDH1A1 through Affinity Capture-MS. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human ALDH1A1 and recombinant human IL21. Briefly, ALDH1A1 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL ALDH1A1 were then transferred to IL21-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-ALDH1A1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of ALDH1A1 and IL21 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of ALDH1A1 with IL21.
- Top Product
- Discover our top product ALDH1A1 Protein
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- Application Notes
- Isoelectric Point: 6.3
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
- Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Target
- ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))
- Abstract
- ALDH1A1 Products
- Synonyms
- ALDC Protein, ALDH-E1 Protein, ALDH1 Protein, ALDH11 Protein, PUMB1 Protein, RALDH1 Protein, AL1A1 Protein, aldc Protein, aldh-e1 Protein, aldh1 Protein, aldh11 Protein, pumb1 Protein, raldh1 Protein, GGADHR Protein, ALDH1A1 Protein, ALDDH Protein, Ahd2 Protein, Aldh1 Protein, Aldh2 Protein, Ahd-2 Protein, Aldh1a2 Protein, E1 Protein, Raldh1 Protein, Aldh1a1 Protein, ALHDII Protein, RALDH 1 Protein, RalDH1 Protein, aldehyde dehydrogenase 1 family member A1 Protein, aldehyde dehydrogenase 1 family, member A1 Protein, aldehyde dehydrogenase 1 family member A1 L homeolog Protein, aldehyde dehydrogenase family 1, subfamily A1 Protein, aldehyde dehydrogenase Protein, retinal dehydrogenase 1 Protein, ALDH1A1 Protein, aldh1a1 Protein, Aldh1a1 Protein, aldh1a1.L Protein, aldH1 Protein, LOC100732581 Protein, LOC101822890 Protein
- Background
- Alternative Names: ALDH11, ALDH1, ALDC, PUMB1, RALDH1, Retinaldehyde Dehydrogenase 1, Aldehyde dehydrogenase, cytosolic, Retinal dehydrogenase 1
- Molecular Weight
- 58kDa
- UniProt
- P00352
- Pathways
- Dopaminergic Neurogenesis
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