Elastase 4 Protein (AA 30-268) (His tag)

Catalog No. ABIN6239862

Product Details

Target: Elastase 4 (CTRC) (Chymotrypsin C (Caldecrin) (CTRC))

Biological Activity: Active

Protein Characteristics: AA 30-268

Origin: Rat

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Elastase 4 protein is labelled with His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Characteristics: Tag location: N-terminal His Tag

Purity: > 95 %

Biological Activity Comment: Elastase 4 (ELA4) is a member of the peptidase S1 family. The encoded protein is a serum calcium-decreasing factor that has chymotrypsin-like protease activity. It regulates activation and degradation of trypsinogens and procarboxypeptidases by targeting specific cleavage sites within their zymogen precursors. Has chymotrypsin-type protease activity and hypocalcemic activity. Besides, Plasminogen Activator, Urokinase Receptor (uPAR) has been identified as an interactor of ELA4, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat ELA4 and recombinant rat uPAR. Briefly, ELA4 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to uPAR-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-ELA4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of ELA4 and uPAR was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of ELA4 with uPAR.

Application Details

Application Notes: Isoelectric Point: 5.9

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Target Details

Target: Elastase 4 (CTRC) (Chymotrypsin C (Caldecrin) (CTRC))

Alternative Name: Elastase 4 (ELA4) (CTRC Products)

Synonyms: CLCR Protein, ELA4 Protein, 1810044E12Rik Protein, bPTLP Protein, chymotrypsin C Protein, chymotrypsin-C Protein, chymotrypsin C (caldecrin) Protein, CTRC Protein, LOC478220 Protein, Ctrc Protein

Background: Alternative Names: CLCR, CTRC, Chymotrypsin C, Caldecrin

Molecular Weight: 30kDa

UniProt: P55091