VISTA Protein (AA 33-191) (His tag)
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- Target See all VISTA Proteins
- VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 33-191
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Origin
- Mouse
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Source
- Human Cells
- Purification tag / Conjugate
- This VISTA protein is labelled with His tag.
- Application
- SDS-PAGE (SDS), ELISA, Western Blotting (WB), Mass Spectrometry (MS)
- Purpose
- Recombinant Mouse VISTA Protein is produced by mammalian expression system and the target gene encoding Phe33-Ala191 is expressed fused with a 6His tag at the C-terminus.
- Sequence
- FKVTTPYSLY VCPEGQNATL TCRILGPVSK GHDVTIYKTW YLSSRGEVQM CKEHRPIRNF TLQHLQHHGS HLKANASHDQ PQKHGLELAS DHHGNFSITL RNVTPRDSGL YCCLVIELKN HHPEQRFYGS MELQVQAGKG SGSTCMASNE QDSDSITAAH HHHHH
- Purity
- Greater than 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Top Product
- Discover our top product VISTA Protein
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V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA) protein (His tag)
VISTA Origin: Cynomolgus Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA) (AA 1-311) protein (GST tag)VISTA Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA) protein (Myc-DYKDDDDK Tag)VISTA Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA) protein (DYKDDDDK-His Tag)VISTA Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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- Target
- VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA))
- Alternative Name
- C10orf54 (VISTA Products)
- Synonyms
- MGC112715 Protein, MGC151567 Protein, B7-H5 Protein, B7H5 Protein, GI24 Protein, PP2135 Protein, SISP1 Protein, Dies1 Protein, VISTA Protein, V-set immunoregulatory receptor Protein, vsir Protein, Vsir Protein, VSIR Protein
- Pathways
- Cancer Immune Checkpoints
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