PDGFB Protein (AA 82-190) (His tag)
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- Target See all PDGFB Proteins
- PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 82-190
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PDGFB protein is labelled with His tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Mass Spectrometry (MS)
- Purpose
- Recombinant Mouse PDGFB Protein is produced by E.coli expression system and the target gene encoding Ser82-Thr190 is expressed with a 6His tag at the C-terminus.
- Sequence
- MSLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVVTPRPVT LEHHHHHH
- Purity
- Greater than 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
- Top Product
- Discover our top product PDGFB Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Lyophilized from a 0.2 μm filtered solution of 4 mM HCl.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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- Target
- PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))
- Alternative Name
- PDGFB (PDGFB Products)
- Synonyms
- PDGF-2 Protein, PDGF2 Protein, SIS Protein, SSV Protein, c-sis Protein, pdgf2 Protein, sis Protein, si:ch211-79m20.1 Protein, si:dkey-261d11.1 Protein, PDGF-B Protein, Sis Protein, platelet derived growth factor subunit B Protein, platelet-derived growth factor beta polypeptide Protein, platelet derived growth factor subunit B S homeolog Protein, platelet-derived growth factor beta polypeptide a Protein, platelet derived growth factor, B polypeptide Protein, PDGFB Protein, pdgfb.S Protein, pdgfba Protein, Pdgfb Protein
- Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
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