CST5 Protein (AA 26-142) (His tag)
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- Target See all CST5 Proteins
- CST5 (Cystatin D (CST5))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 26-142
- Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This CST5 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- QSRTLAGGIH ATDLNDKSVQ RALDFAISEY NKVINKDEYY SRPLQVMAAY QQIVGGVNYY FNVKFGRTTC TKSQPNLDNC PFNDQPKLKE EEFCSFQINE VPWEDKISIL NYKCRKVHHH HHH
- Purity
- > 90 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- The IC50 value is < 2.0nM. The inhibitory function of Cystatin 5 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.
- Top Product
- Discover our top product CST5 Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In phosphate buffered saline ( pH 7.4) containing 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- CST5 (Cystatin D (CST5))
- Alternative Name
- CST5 (CST5 Products)
- Synonyms
- Cst10 Protein, Cystatin-5 Protein, cystatin D Protein, CST5 Protein, Cst5 Protein
- Background
- CST5, also known as Cystatin-D, is a novel member of the cystatin superfamily. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. Two alleles of the cystatin D gene (CST5), encoding protein variants with either Cys or Arg as residue 26 in their 122-residue polypeptide chains, are present in the population. Recombinant human CST5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 14.36kDa (123aa) (13.5-18kDa in SDS-PAGE under reducing conditions.)
- NCBI Accession
- NP_001891
- UniProt
- P28325
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