HLA-DRA Protein (AA 26-216) (His tag)
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- Target See all HLA-DRA Proteins
- HLA-DRA (HLA Class II DR alpha (HLA-DRA))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 26-216
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This HLA-DRA protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MIKEEHVIIQ AEFYLNPDQS GEFMFDFDGD EIFHVDMAKK ETVWRLEEFG RFASFEAQGA LANIAVDKAN LEIMTKRSNY TPITNVPPEV TVLTNSPVEL REPNVLICFI DKFTPPVVNV TWLRNGKPVT TGVSETVFLP REDHLFRKFH YLPFLPSTED VYDCRVEHWG LDEPLLKHWE FDAPSPLPET TE
- Purity
- > 90 % by SDS - PAGE
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HLA Class II DR alpha (HLA-DRA) protein
HLA-DRA Origin: Dog Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, WB, ELISA, IP
HLA Class II DR alpha (HLA-DRA) proteinHLA-DRA Origin: Pig Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, WB, ELISA, IP
HLA Class II DR alpha (HLA-DRA) proteinHLA-DRA Origin: Cow Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, WB, ELISA, IP
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- HLA-DRA (HLA Class II DR alpha (HLA-DRA))
- Alternative Name
- HLA-DRA (HLA-DRA Products)
- Synonyms
- HLA-DRA1 Protein, MLRW Protein, DR-alpha Protein, HLA-DRA Protein, Mamu-DRA1 Protein, major histocompatibility complex, class II, DR alpha L homeolog Protein, major histocompatibility complex, class II, DR alpha Protein, hla-dra.L Protein, HLA-DRA Protein, MAMU-DRA Protein, BOLA-DRA Protein
- Background
- Major histocompatibility complex, class II, DR alpha, also known as HLA-DRA, binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Recombinant human HLA-DRA, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 24.3 kDa (212aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_061984
- UniProt
- P01903
- Pathways
- TCR Signaling, CXCR4-mediated Signaling Events, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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