Tubulin Protein (TUB)
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- Target See all Tubulin (TUB) Proteins
- Tubulin (TUB)
- Protein Type
- Native
- Origin
- Cow
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Source
- Cow
- Application
- Biochemical Assay (BCA), Crystallization (Crys), Electron Microscopy (EM), High-throughput Screening (HTS)
- Purpose
- Endogenous cytoskeletal protein
- Characteristics
- Polymerizes to form microtubules
- Purification
- Endogenous protein purified by differential centrifugation
- Purity
- > 99 %
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- Application Notes
- Tested for purity (protein gel) and polymerization competency (fluorescence microscopy and light scattering)
- Comment
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for use, but not limited to, in cell-free experimental systems including: structural analysis by X-ray crystallography and electron, drug discovery by high-throughput screening microscopy, in vitro biochemical and biophysical approaches
- Protocol
- The recommended tubulin concentration for ensuring polymerization is 2 mg/mL
- Restrictions
- For Research Use only
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- Format
- Frozen
- Reconstitution
- Thaw rapidly at 37 °C and immediately place on ice
- Concentration
- 20 mg/mL
- Buffer
- 80 mM PIPES, 1 mM EGTA, and 1 mM MgCl2, pH 6.8
- Preservative
- Without preservative
- Storage
- -80 °C
- Storage Comment
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Storage: -80°C
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- Target
- Tubulin (TUB)
- Alternative Name
- tubulin (TUB Products)
- Synonyms
- 4t Protein, B4t Protein, BETA 98B Protein, CG4869 Protein, DTB1 Protein, Dmbeta4 Protein, Dmel\\CG4869 Protein, T Protein, Tub Protein, beta-Tub Protein, beta-Tub97EF Protein, beta-tub Protein, beta4 Protein, beta4t Protein, beta97F Protein, betaTub4 Protein, betaTub98BC Protein, betaTub98C Protein, beta-Tubulin at 97EF Protein, betaTub97EF Protein
- Molecular Weight
- ~110 kDa
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