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- Target See all PARP1 Proteins
- PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))
- Protein Characteristics
- N-Term
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- Control (Ct)
- Purification
- PARP1 (N-term ZF1) is an N-terminus His-Tag recombinant protein expressed in E.coli that corresponds to a fragment of the human PARP1 zinc finger domain. Analysis by SDS-PAGE and Coomassie staining resulted in ~13 kDa MW band and estimated purity ≥95%.
- Sterility
- Sterile filtered
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Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 662-1014) protein
PARP1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE SDS
Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 1-100) protein (GST tag)PARP1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Poly (ADP-Ribose) Polymerase 1 (PARP1) (Active) protein (His tag)PARP1 Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 85 % as determined by SDS-PAGE Active
Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 2-331), (partial) protein (His tag)PARP1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Poly (ADP-Ribose) Polymerase 1 (PARP1) protein (DYKDDDDK Tag)PARP1 Origin: Human Host: Insect cells (Sf9) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Application Note: PARP1 (N-term ZF1) is suitable as a control for immunological assays that use Anti-PARP1-ZF (RABBIT) Antibody (200-401-GM8). Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13 kDa in size corresponding to PARP-1 by western blotting.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 % (v/v) Glycerol
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable 2 - 3 weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))
- Alternative Name
- PARP1 (PARP1 Products)
- UniProt
- P09874
- Pathways
- Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location
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