MEK2 Protein (pSer222, pSer226)

Catalog No. ABIN5624616

Product Details

Target: MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))

Protein Type: Recombinant

Protein Characteristics: pSer222, pSer226

Origin: Human, Mouse, Rat

Source: Insect cells (Sf9)

Application: Western Blotting (WB)

Purification: MEK2 pS222-pS226 is a recombinant protein containing a polyhistidine tag expressed in Sf9. This protein is expressed in the presense of constitutively active B-raf (V600E) leading to high levels of phosphorylation of MEK2 activation residues S222 and S226. Analysis by SDS-PAGE resulted in a pattern consistent with purified MEK2 and was estimated to be greater than 95% pure.

Sterility: Sterile filtered

Application Details

Application Notes:

Application Note: MEK2 pS222-pS226 is an activated recombinant protein suitable as a control for polyclonal or monoclonal anti-MEK2 in immunological assays. Activated MEK1 can be used in vitro to phosphorylate ERK protein. For western blot use at 50 ng or less. For other assays concentration is user optimized.

Western Blot Dilution: 50 ng

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Stabilizer: None

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))

Alternative Name: MEK2 (MAP2K2 Products)

Synonyms: CFC4 Protein, MAPKK2 Protein, MEK2 Protein, MKK2 Protein, PRKMK2 Protein, AA589381 Protein, MK2 Protein, Prkmk2 Protein, ATMKK2 Protein, F27B13.50 Protein, F27B13_50 Protein, MAP KINASE KINASE 1 Protein, MAP kinase kinase 2 Protein, MK1 Protein, map2k2 Protein, wu:fa56c06 Protein, zgc:123171 Protein, zgc:172250 Protein, mitogen-activated protein kinase kinase 2 Protein, mitogen activated protein kinase kinase 2 Protein, MAP kinase kinase 2 Protein, mitogen-activated protein kinase kinase 2a Protein, mitogen-activated protein kinase kinase 2b Protein, MAP2K2 Protein, Map2k2 Protein, MKK2 Protein, map2k2a Protein, map2k2b Protein

UniProt: P36507

Pathways: MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling