MEK2 Protein (double Mutant)

Catalog No. ABIN5624614

Product Details

Target: MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))

Protein Type: Recombinant

Protein Characteristics: double Mutant

Origin: Human, Mouse, Rat

Source: Insect cells (Sf9)

Application: Western Blotting (WB)

Purification: MEK2 double mutant human is a recombinant protein containing a polyhistidine tag expressed in Sf9. This double mutant protein contain alanine substitutions at S222A and S226A rendering it inactive. Analysis by SDS-PAGE resulted in a pattern consistent with purified MEK2 and was estimated to be greater than 95% pure.

Sterility: Sterile filtered

Application Details

Application Notes:

Application Note: MEK2 mutant protein is suitable as a negative control protein for immunoassays using antibodies targeting the critical MEK2 phosphorylated S222 or S226 residues. For western blot use at 50 ng or less. For other assays concentration is user optimized.

Western Blot Dilution: 50 ng

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Stabilizer: None

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))

Alternative Name: MEK2 (MAP2K2 Products)

Synonyms: CFC4 Protein, MAPKK2 Protein, MEK2 Protein, MKK2 Protein, PRKMK2 Protein, AA589381 Protein, MK2 Protein, Prkmk2 Protein, ATMKK2 Protein, F27B13.50 Protein, F27B13_50 Protein, MAP KINASE KINASE 1 Protein, MAP kinase kinase 2 Protein, MK1 Protein, map2k2 Protein, wu:fa56c06 Protein, zgc:123171 Protein, zgc:172250 Protein, mitogen-activated protein kinase kinase 2 Protein, mitogen activated protein kinase kinase 2 Protein, MAP kinase kinase 2 Protein, mitogen-activated protein kinase kinase 2a Protein, mitogen-activated protein kinase kinase 2b Protein, MAP2K2 Protein, Map2k2 Protein, MKK2 Protein, map2k2a Protein, map2k2b Protein

UniProt: P36507

Pathways: MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling