MEK1 Protein

Catalog No. ABIN5624610

Supplier Product No.: 009-001-gq3

Product Details

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Application: Western Blotting (WB), SDS-PAGE (SDS)

Supplier Product No.: 009-001-gq3

Supplier: Rockland

Purpose: MEK1 human recombinant protein

Characteristics: MAP2K, MEK, MEK1, MKK1, PRKMK1, MEK-1 recombinant protein

Purification: MEK1 is a recombinant protein containing a polyhistidine tag expressed in Sf9. Analysis by SDS-PAGE resulted in a pattern consistent with purified MEK1 and was estimated to be greater than 90% pure.

Sterility: Sterile filtered

Application Details

Application Notes:

Application Note: Human MEK1 recombinant protein has been tested in SDS-Page and is suitable as a control for polyclonal or monoclonal anti-MEK1 in immunological assays. For western blot use at 50 ng or less. For other assays concentration is user optimized. For a phosphorylated form of the protein co-expressed with constitutively active B-raf (V600E) see (p/n 009-001-GP5) MEK1 pS218-pS222.

Western Blot Dilution: 50 ng

Other: Dot Blot

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Alternative Name: MAP2K1 (MAP2K1 Products)

Background: Background: MEK1 recombinant protein was produced in Sf9 cells. Mitogen-activated protein kinase kinase 1, also known as MKK or MEK, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. MEK1 and MEK2 are about 80 % identical to each other, and nearly identical within the kinase domain. Recombinant MEK1 protein is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.

UniProt: Q02750

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling