MEK1 Protein (double Mutant)

Catalog No. ABIN5624609

Product Details

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Protein Type: Recombinant

Protein Characteristics: double Mutant

Origin: Human, Mouse, Rat

Source: Insect cells (Sf9)

Application: Western Blotting (WB)

Purification: MEK1 double mutant human is a recombinant protein containing a polyhistidine tag expressed in Sf9. This double mutant protein contain alanine substitutions at S218A and S222A rendering it inactive. Analysis by SDS-PAGE resulted in a pattern consistent with purified MEK1 and was estimated to be greater than 95% pure.

Sterility: Sterile filtered

Application Details

Application Notes:

Application Note: MEK1 mutant protein is suitable as a negative control protein for immunoassays using antibodies targeting the critical MEK1 phosphorylated S218 or S222 residues. For western blot use at 50 ng or less. For other assays concentration is user optimized.

Western Blot Dilution: 50 ng

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Stabilizer: None

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Alternative Name: MEK1 (MAP2K1 Products)

Synonyms: Mek1 Protein, CFC3 Protein, MAPKK1 Protein, MEK1 Protein, MKK1 Protein, PRKMK1 Protein, MEKK1 Protein, Prkmk1 Protein, mek-2 Protein, si:ch211-242m18.2 Protein, wu:fj56a12 Protein, wu:fj61b01 Protein, zgc:56557 Protein, ATMEK1 Protein, F20B18.180 Protein, F20B18_180 Protein, MAP kinase/ ERK kinase 1 Protein, MITOGEN ACTIVATED PROTEIN KINASE KINASE Protein, MITOGEN ACTIVATED PROTEIN KINASE KINASE 1 Protein, NMAPKK Protein, MEK Protein, mitogen activated protein kinase kinase 1 Protein, mitogen-activated protein kinase kinase 1 Protein, mitogen-activated protein kinase kinase 1 L homeolog Protein, MAP kinase/ ERK kinase 1 Protein, Map2k1 Protein, MAP2K1 Protein, map2k1.L Protein, map2k1 Protein, MEK1 Protein

UniProt: Q02750

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling