MEK1 Protein (double Mutant)

Catalog No. ABIN5624609

Product Details

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Protein Type: Recombinant

Protein Characteristics: double Mutant

Origin: Human

Source: Insect cells (Sf9)

Application: Western Blotting (WB), SDS-PAGE (SDS)

Purpose: MEK1 double mutant human recombinant protein

Characteristics: MAP2K, MEK, MEK1, MKK1, PRKMK1, MEK-1 recombinant protein

Purification: MEK1 double mutant human is a recombinant protein containing a polyhistidine tag expressed in Sf9. This double mutant protein contain alanine substitutions at S218A and S222A rendering it inactive. Analysis by SDS-PAGE resulted in a pattern consistent with purified MEK1 and was estimated to be greater than 90% pure.

Sterility: Sterile filtered

Application Details

Application Notes:

Application Note: Human MEK1 mutant recombinant protein has been tested in SDS-Page and is suitable as a negative control protein for immunoassays using antibodies targeting the critical MEK1 phosphorylated S218 or S222 residues. For western blot use at 50 ng or less. For other assays concentration is user optimized.

Western Blot Dilution: 50 ng

Other: Dot Blot

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Alternative Name: MAP2K1 (MAP2K1 Products)

Background: Background: MEK1 isoform double mutant recombinant protein is modified to put alanine residues at two key activation sites, Serine 218 and Serine 222. Mitogen-activated protein kinase kinase 1, also known as MKK or MEK, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. The inactive double mutant MEK1 recombinant protein is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.

UniProt: Q02750

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling