Flagellin Protein (FliC)

Catalog No. ABIN5624606

Product Details

Target: Flagellin (FliC)

Protein Type: Recombinant

Origin: Borrelia burgdorferi

Source: Escherichia coli (E. coli)

Application: ELISA, Western Blotting (WB)

Purification: Flagellin is a fusion protein with an MBP tag and was expressed in E.coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified Flagellin and was estimated to be greater than 95% pure.

Sterility: Sterile filtered

Application Details

Application Notes:

Application Note: Flagellin is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 76.3 kDa Flagellin-MBP, (33.9 kDa for Flagellin and 42.4 kDa for MBP) in size corresponding to Flagellin by Western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: User Optimized

ELISA Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

Expiry Date: 6 months

Target Details

Target: Flagellin (FliC)

Alternative Name: Flagellin (FliC Products)

Synonyms: hag Protein, flaG Protein, flagellin Protein, flagellin (flic) Protein, flagellin E Protein, flagellin protein flic Protein, fliC Protein, FliC Protein, fliC+F2048 Protein, CJJ81176_1339 Protein, C8J_1256 Protein

Gene ID: 7106737

UniProt: P11089

Pathways: Inflammasome