ERK1 Protein (pThr202, pTyr204)

Catalog No. ABIN5624590

Product Details

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Protein Type: Recombinant

Protein Characteristics: pThr202, pTyr204

Origin: Human, Rat, Mouse

Source: Insect cells (Sf9)

Application: Western Blotting (WB)

Purification: ERK1 pT202-pY204 is a recombinant protein containing a polyhistidine tag expressed in Sf9. ERK1 was phosphorylated in vitro using the activated MEK. Analysis by SDS-PAGE resulted in a pattern consistent with purified ERK1 and was estimated to be greater than 95% pure.

Sterility: Sterile filtered

Application Details

Application Notes:

Application Note: ERK1 pT202-pY204 is an activated recombinant protein suitable as a control for polyclonal or monoclonal anti-ERK in immunological assays. ERK1 can be activated by in vitro phosphorylation by MEK proteins. For western blot use at 50 ng or less. For other assays concentration is user optimized.

Western Blot Dilution: 50 ng

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Stabilizer: None

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Alternative Name: ERK1 (MAPK3 Products)

Synonyms: ERK-1 Protein, ERK1 Protein, ERT2 Protein, HS44KDAP Protein, HUMKER1A Protein, P44ERK1 Protein, P44MAPK Protein, PRKM3 Protein, p44-ERK1 Protein, p44-MAPK Protein, Erk-1 Protein, Erk1 Protein, Ert2 Protein, Esrk1 Protein, Mnk1 Protein, Mtap2k Protein, Prkm3 Protein, p44 Protein, p44erk1 Protein, p44mapk Protein, ERK3 Protein, ERK6 Protein, P38GAMMA Protein, PRKM12 Protein, SAPK-3 Protein, SAPK3 Protein, fi06b09 Protein, wu:fi06b09 Protein, zERK1 Protein, Tb08.10J17.940 Protein, MAPK1 Protein, MNK1 Protein, AW123708 Protein, Erk6 Protein, P38gamma Protein, Prkm12 Protein, Sapk3 Protein, ATMAPK3 Protein, ATMPK3 Protein, T6D9.4 Protein, mitogen-activated protein kinase 3 Protein, mitogen-activated protein kinase 3 Protein, mitogen-activated protein kinase 12 Protein, mitogen activated protein kinase 3 Protein, mitogen-activated serine/threonine-protein kinase Protein, MAPK3 Protein, Mapk3 Protein, MAPK12 Protein, mapk3 Protein, Tc00.1047053509475.10 Protein, Tb927.8.3550 Protein, Mapk12 Protein, CEK1 Protein, MPK3 Protein

UniProt: P27361

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins