ERK1 Protein (double Mutant)

Catalog No. ABIN5624588

Supplier Product No.: 009-001-gr1

Product Details

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Protein Type: Recombinant

Protein Characteristics: double Mutant

Origin: Human

Source: Insect cells (Sf9)

Application: Western Blotting (WB), SDS-PAGE (SDS)

Supplier Product No.: 009-001-gr1

Supplier: Rockland

Purpose: ERK1 double mutant human recombinant protein

Characteristics: MAPK3, ERK, ERK1, PRKM3, ERK-1 recombinant protein

Purification: ERK1 double mutant human is a recombinant protein containing a polyhistidine tag expressed in Sf9. Analysis by SDS-PAGE resulted in a pattern consistent with purified ERK1 and was estimated to be greater than 90% pure.

Sterility: Sterile filtered

Application Details

Application Notes:

Application Note: Human ERK1 mutant recombinant protein has been tested in SDS-Page and is suitable as a negative control protein for immunoassays using antibodies targeting the critical ERK1 phosphorylated T202 or Y204 residues. For western blot use at 50 ng or less. For other assays concentration is user optimized.

Western Blot Dilution: 50 ng

Other: Dot Blot

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Alternative Name: MAPK3 (MAPK3 Products)

Background: Background: ERK1 isoform double mutant recombinant protein is modified to put alanine residues at two key activation sites, Threonine 202 and Tyrosine 204. Mitogen activated protein kinase 3, also known as MAPK3, ERK, or ERK1, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. The inactive double mutant ERK1 recombinant protein is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.

UniProt: P27361

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins