OspB Protein
Rockland 000-001-c15
Origin: Borrelia burgdorferi
Host: Escherichia coli (E. coli)
Recombinant
ELISA, WB
1 image
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- Target
- OspB
- Protein Type
- Recombinant
- Origin
- Borrelia burgdorferi
- Source
- Escherichia coli (E. coli)
- Application
- ELISA, Western Blotting (WB)
- Supplier Product No.
- 000-001-c15
- Supplier
- Rockland
- Purification
- OspB is a fusion protein with an MBP tag and was expressed in E.coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified OspB and was estimated to be greater than 95% pure.
- Sterility
- Sterile filtered
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- Application Notes
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Application Note: OspB is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 72.7 kDa for OspB-MBP, (30.3 kDa for OspB and 42.4 for MBP) in size corresponding to OspB by Western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
- Expiry Date
- 6 months
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- Target
- OspB
- Gene ID
- 1194340
- UniProt
- P17739
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