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OspA Protein

OspA Origin: Borrelia burgdorferi Host: Escherichia coli (E. coli) Recombinant ELISA, WB
Catalog No. ABIN5624575
  • Target See all OspA products
    OspA (Borrelia Burgdorferi Outer Surface Protein A (OspA))
    Protein Type
    Recombinant
    Origin
    Borrelia burgdorferi
    Source
    Escherichia coli (E. coli)
    Application
    ELISA, Western Blotting (WB)
    Purification
    OspA is a fusion protein with an MBP tag and was expressed in E.coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified OspA and was estimated to be greater than 95% pure.
    Sterility
    Sterile filtered
  • Application Notes

    Application Note: OspA is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 70.5 kDa for OspA-MBP, (28.1 kDa for OspA and 42.4 for MBP) in size corresponding to OspA by Western blotting in the appropriate cell lysate or extract.

    Western Blot Dilution: User Optimized

    ELISA Dilution: User Optimized

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.04 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
    Expiry Date
    6 months
  • Target
    OspA (Borrelia Burgdorferi Outer Surface Protein A (OspA))
    Alternative Name
    OspA (OspA Products)
    Gene ID
    1194357
    UniProt
    P0C926
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